cell
Tissue
ELISA
Verified applications
11082
Gene number
Q9NQ30
Protein number
Endocan
Alternate gene name
3 hours
Assay duration
Sandwich
Assay class
8 months
Use for
Blue ice
Shipping requirements
6,2 pg/mL
Sensitivity limit
Colorimetric
ELISA detection
15.6-1000 pg/mL
Detection limits
7-11 business days
Estimated production time
Homo sapiens (Human)
Verified reactivity
Cardiovascular biology;
Research main area
Please refer to SwissProt
Alternate protein number
Endothelial Cell Specific Molecule 1
Gene name
Please see ELISA's datasheet, otherwise contact us
Protocol
For research use only. Not for diagnostic procedures.
Notes
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Test
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
QC
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Samples to be analyzed
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
ELISA's cross-reactivity
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Storage recommendation
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Warnings
This assay has high sensitivity and excellent specificity for detection of Endothelial Cell Specific Molecule 1 (ESM1). No significant cross-reactivity or interference between Endothelial Cell Specific Molecule 1 (ESM1) and analogues was observed.
ELISA's specificity
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelial Cell Specific Molecule 1 (ESM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelial Cell Specific Molecule 1 (ESM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Precision of the test
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ELISA's stability
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Properties
For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial Cell Specific Molecule 1 (ESM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelial Cell Specific Molecule 1 (ESM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay principle