cell
Tissue
2674
Gene number
ELISA
Verified applications
P56159
Protein number
3 hours
Assay duration
Blue ice
Shipping requirements
Sandwich
Assay class
8 months
Use for
0,119 ng/mL
Sensitivity limit
Colorimetric
ELISA detection
0.312-20 ng/mL
Detection limits
7-11 business days
Estimated production time
Infection immunity;
Research main area
Homo sapiens (Human)
Verified reactivity
Please refer to SwissProt
Alternate protein number
Tissue homogenates and other biological fluids.
Samples to be analyzed
Please see ELISA's datasheet, otherwise contact us
Protocol
For research use only. Not for diagnostic procedures.
Notes
Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1
Gene name
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Test
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
QC
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
ELISA's cross-reactivity
GDNFR; GDNFRA; GFR-ALPHA-1; RET1L; RETL1; TRNR1; GDNF family receptor alpha-1; TGF-beta-related neurotrophic factor receptor 1
Alternate gene name
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Storage recommendation
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Warnings
This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) and analogues was observed.
ELISA's specificity
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Precision of the test
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ELISA's stability
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Properties
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay principle
The Glial Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) ELISA Kit, 96 is a α- or alpha protein sometimes glycoprotein present in blood.For cells, cell lines and tissues in culture till half confluency.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Description