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  • Mouse Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit, 96 well plate

    Size

    10x96T

    Catalog no.

    EKU08077

    Price

    4124 EUR

    Example photo
    Mouse Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit, 96 well plate

    Buy at gentaur.com

    ELISA

    Verified applications

    P29533

    Protein number

    3 hours

    Assay duration

    8 months

    Use for

    Sandwich

    Assay class

    Blue ice

    Shipping requirements

    0,15 ng/mL

    Sensitivity limit

    Colorimetric

    ELISA detection

    Mus musculus

    Latin name

    cell, vascular

    Tissue

    0.391-25 ng/mL

    Detection limits

    7-11 business days

    Estimated production time

    Mus musculus (Mouse)

    Verified reactivity

    Please refer to GenBank

    Gene number

    CD106; INCAM-100; L1CAM

    Alternate gene name

    Please refer to SwissProt

    Alternate protein number

    Vascular Cell Adhesion Molecule 1

    Gene name

    Please see ELISA's datasheet, otherwise contact us

    Protocol

    For research use only. Not for diagnostic procedures.

    Notes

    CD & Adhesion molecule;Tumor immunity;Infection immunity;

    Research main area

    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

    QC

    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

    Samples to be analyzed

    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

    Properties

    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

    ELISA's cross-reactivity

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    Storage recommendation

    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    Warnings

    This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1). No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.

    ELISA's specificity

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

    Precision of the test

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    ELISA's stability

    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

    Test

    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Assay principle

    cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Mouse Vascular Adhesion Molecule 1 (VCAM1) ELISA Kit, 96.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

    Description

    CellCycl

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