3 hours
6.3 pg/mL
Ice packs
cell, stem
Colorimetric
15.6-1000 pg/mL
Stem Cell Factor
Rattus norvegicus
6-11 Business days
Cytokine, Hematology
Rattus norvegicus (Rat)
Double-antibody Sandwich
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
Use Rat Stem Cell Factor (SCF) ELISA Kit before 8 months
The Stop Solution is acidic. Do not allow to contact skin or eyes.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
No significant cross-reactivity or interference between Stem Cell Factor and analogues was observed.
No significant cross-reactivity or interference between Stem Cell Factor and analogues was observed.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For cells, cell lines and tissues in culture till half confluency.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.