3 hours
Assay Time
Ice packs
Shipping Conditions
0.33 ng/mL
Sensitivity
Colorimetric
Detection Method
0.78-50 ng/mL
Detection Range
cell, vascular
Tissue
6-11 Business days
Estimated Turnaround Time
Oryctolagus cuniculus
Latin name
CD106, INCAM-100, L1CAM
Target's alterntive name
Double-antibody Sandwich
Assay Type
Oryctolagus cuniculus (Rabbit)
Species Reactivity
Vascular Cell Adhesion Molecule 1
Target Name
Short term: 4°C; Long term: see manual.
Storage Temperature
For research use only. Not for diagnostic procedures.
Application
CD & Adhesion molecule, Tumor immunity, Infection immunity
Research Area
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Test
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Precaution of Use
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Systems
Use Rabbit Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit before 8 months
Shelf life
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Sample Type
This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1
Specificity
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Properties
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 and analogues was observed.
Cross Activity
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 and analogues was observed.
Test Principle
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Rabbit Vascular Adhesion Molecule 1 (VCAM1) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Description
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Precision
Rabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
About
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Stability