Kit
41116158
Metabolism Research
2 parts: 1 RT / 1 Blue Ice
Detection sensitivity limit of 10ng/mL Homocysteine-BSA
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Suitable for use with plasma, serum, lysates, and other biological fluid samples
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
(STA-670) GHS.pdf
Upon receipt, store Homocysteine Conjugate (1000X) and Homocysteine-BSA Standard at -20ºC. Store the rest of the kit at 4ºC.
Homocysteine is an amino acid intermediate that is synthesized from methionine via S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. Our Homocysteine ELISA is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-homocysteine antibody are added to a plate coated with a homocysteine conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high homocysteine levels will bind to most of the antibody, which gets washed away and results in a low OD, while samples with low homocysteine levels will leave more antibody available to bind to the conjugate, producing a high signal.