Kit
41116158
Viral Expression
2 parts: 1 RT / 1 Blue Ice
Lentivirus quantitation on a standard microplate reader
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Proprietary technology separates virus-associated p24 from free p24
Minimizes overestimation of lentivirus titer common to traditional p24 ELISA kits
(VPK-107) GHS.pdf
Upon receipt, aliquot and store the Recombinant HIV-1 p24 Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present. Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.