cell

Tissue

ELISA

Tested for

Blue Ice

Shipping conditions

Research kit

Category

Store at 2-8 deg C

Storage

Nutrition & Metabolism

Area of research

Optimal conditions to be determined by end-user

Usage Recommendations

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Test

For cells, cell lines and tissues in culture till half confluency.

Description

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Properties

AntiParietal Cell (H+/K+ATPase) is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against the a and bsubunits of the Parietal Cell H+/K+ATPase. Preparations of highly purified pig Parietal Cell H+/K+ATPase are coated on microplates. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). The binding of present autoantibodies, formation of the sandwich complexes and enzymatic colour reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended.

Assay Information