cell
3 hours
Ice packs
0.062 ng/mL
Colorimetric
0.156-10 ng/mL
Enzyme & Kinase
Rattus norvegicus
6-11 Business days
Chymase 1, Mast Cell
Rattus norvegicus (Rat)
Double-antibody Sandwich
Short term: 4°C; Long term: see manual.
CYH, CYM, Alpha-chymase, Mast cell protease I
For research use only. Not for diagnostic procedures.
Use Rat Chymase 1, Mast Cell (CMA1) ELISA Kit before 8 months
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
For cells, cell lines and tissues in culture till half confluency.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Serum, Plasma, Tissue homogenates, Cell lysates and other biological fluids
This assay has high sensitivity and excellent specificity for detection of Chymase 1, Mast Cell
No significant cross-reactivity or interference between Chymase 1, Mast Cell and analogues was observed.
No significant cross-reactivity or interference between Chymase 1, Mast Cell and analogues was observed.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chymase 1, Mast Cell were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chymase 1, Mast Cell were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.