3 hours
Ice packs
0.45 ng/mL
Colorimetric
1.25-80 ng/mL
cell, vascular
Rattus norvegicus
6-11 Business days
CD106, INCAM-100, L1CAM
Rattus norvegicus (Rat)
Double-antibody Sandwich
Vascular Cell Adhesion Molecule 1
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
CD & Adhesion molecule, Tumor immunity, Infection immunity
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
The Stop Solution is acidic. Do not allow to contact skin or eyes.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Use Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit before 8 months
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 and analogues was observed.
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 and analogues was observed.
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Vascular Adhesion Molecule 1 (VCAM1) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.