NA
NA
NA
NA
Rat
cell
Sandwich
4.5 hours
Ice packs
BAFF/CD257
37.5 pg/mL
Colorimetric
62.5-4000 pg/mL
Rattus norvegicus
6-11 business days
Cancer, Cell Biology, Immunology
Serum, Plasma, Biological Fluids
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
The kit is manufactured at ISO 9001 certified facilities.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
No significant cross-reactivity or interference was observed.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Use Rat BAFF/CD257 (B-Cell Activating Factor) ELISA Kit before 6 months
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
For cells, cell lines and tissues in culture till half confluency.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BAFF/CD257 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BAFF/CD257. The concentration of Rat BAFF/CD257 in samples can be calculated by comparing the OD of the samples to the standard curve.