heparin or EDTA
156pg/ml to 10000pg/ml
Basal cell adhesion molecule
Natural and recombinant human BCAM
basal cell adhesion molecule (Lutheran blood group)
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
AU ; Bcam ; CD239 ; CD239 antigen ; F8/G253 antigen ; LU ; Lutheran ; MSK19 ; P50895
the ELISA does not cross react with any other proteins except from closely related species
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
For quantitative measurement of human BCAM in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.067 + 0.125 + 0.175 + 0.250 + 0.450 + 0.848 + 1.489 + 2.153
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);436;729;1325;592;886;1472 ELISA standard deviations 24.41;49.57;86.12;40.85;69.1;125.12 CV(%);5.6;6.8;6.5;6.9;7.8;8.5
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human BCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each human BCAM standard solution and each sample be measured in duplicate.
Catalog number;Description;Quantity -;96-well plate precoated with anti- human BCAM antibodies;1 ST0000-10;Lyophilized recombinant human BCAM standard;10ng/tube×2 AR1107;Biotinylated anti- human BCAM antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
GENTAUR's human BCAM ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for BCAM was used as capure antibodies on the ELISA test plate. The internal standards (Expression system for standard: NSO, Immunogen sequence: E32-A547) and your samples are measured with a biotin conjugated polyclonal from goat specific for BCAM will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human BCAM in your sample the higher the ELISA signal will be compared to this elisa's standard curve.