heparin or EDTA
156pg/ml to 10000pg/ml
cell adhesion molecule L1 like
Natural and recombinant human CHL1
Neural cell adhesion molecule L1-like protein
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
the ELISA does not cross react with any other proteins except from closely related species
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
For quantitative measurement of human CHL1 in cell culture supernatant , cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.018 + 0.169 + 0.232 + 0.306 + 0.502 + 0.853 + 1.462 + 2.193
CALL ; Cell adhesion molecule with homology to L1CAM (close homolog of L1) ; Close homolog of L1 ; FLJ44930 ; L1 cell adhesion molecule 2 ; L1CAM2 ; MGC132578 ; Neural cell adhesion molecule ; O00533
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);223;1080;4453;195;957;3957 ELISA standard deviations 15.39;69.12;289.45;7.99;50.72;265.12 CV(%);6.9;6.4;6.5;4.1;5.3;6.7
Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.
Catalog number;Description;Quantity -;96-well plate precoated with anti- human CHL1 antibodies;1 ST0000-10;Lyophilized recombinant human CHL1 standard;10ng/tube×2 AR1107;Biotinylated anti- human CHL1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human CHL1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each human CHL1 standard solution and each sample be measured in duplicate.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
GENTAUR's human CHL1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CHL1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: I25-Q1096) and your samples are measured with a biotin conjugated polyclonal from goat specific for CHL1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human CHL1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.