cell
3 hours
Ice packs
0.253 ng/mL
Mus musculus
Colorimetric
0.625-40 ng/mL
6-11 Business days
Mus musculus (Mouse)
Double-antibody Sandwich
Melanoma Cell Adhesion Molecule
Short term: 4°C; Long term: see manual.
CD & Adhesion molecule, Tumor immunity, Dermatology
For research use only. Not for diagnostic procedures.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Use Mouse Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit before 8 months
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
This assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule and analogues was observed.
No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule and analogues was observed.
CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Mouse Melanoma Adhesion Molecule (MCAM) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.