cell
Cyclin
3 hours
Ice packs
0.055 ng/mL
Colorimetric
0.156-10 ng/mL
6-11 Business days
Double-antibody Sandwich
Proliferating Cell Nuclear Antigen
Tumor immunity, Developmental science
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Use Multi-species Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit before 8 months
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
This assay has high sensitivity and excellent specificity for detection of Proliferating Cell Nuclear Antigen
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
No significant cross-reactivity or interference between Proliferating Cell Nuclear Antigen and analogues was observed.
No significant cross-reactivity or interference between Proliferating Cell Nuclear Antigen and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proliferating Cell Nuclear Antigen were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proliferating Cell Nuclear Antigen were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.