NA
NA
NA
NA
cell
Human
Sandwich
4.5 hours
Ice packs
L1CAM/CD171
56.25 pg/mL
Colorimetric
93.75-6000 pg/mL
6-11 business days
Serum, Plasma, Biological Fluids
Short term: 4°C; Long term: see manual.
Cancer, Neuroscience, Signal transduction
For research use only. Not for diagnostic procedures.
The kit is manufactured at ISO 9001 certified facilities.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
No significant cross-reactivity or interference was observed.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Use Human L1CAM/CD171 (L1-Cell Adhesion Molecule) ELISA Kit before 6 months
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the L1CAM/CD171 (L1- Adhesion Molecule) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human L1CAM/CD171 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human L1CAM/CD171 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human L1CAM/CD171, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human L1CAM/CD171. The concentration of Human L1CAM/CD171 in samples can be calculated by comparing the OD of the samples to the standard curve.