NA
NA
NA
NA
Mouse
Sandwich
0.1 ng/mL
4.5 hours
Ice packs
Mus musculus
Colorimetric
sVCAM-1/CD106
0.16-10 ng/mL
cell, vascular
6-11 business days
Serum, Plasma, Biological Fluids
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
The kit is manufactured at ISO 9001 certified facilities.
No significant cross-reactivity or interference was observed.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Cancer, Cardiovascular, Neuroscience, Signal transduction, Stem cells
Use Mouse sVCAM-1/CD106 (soluble Vascular Cell Adhesion Molecule 1) ELISA Kit before 6 months
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Mouse sVCAM-1/CD106 (soluble Vascular Adhesion Molecule 1) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sVCAM-1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sVCAM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sVCAM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sVCAM-1. The concentration of Mouse sVCAM-1 in samples can be calculated by comparing the OD of the samples to the standard curve.