NA
NA
NA
NA
PCNA
cell
Rabbit
Sandwich
4.5 hours
Ice packs
0.94 ng/mL
Colorimetric
1.56-100 ng/mL
6-11 business days
Oryctolagus cuniculus
Serum, Plasma, Biological Fluids
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
The kit is manufactured at ISO 9001 certified facilities.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
No significant cross-reactivity or interference was observed.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags & Cell Markers
Use Rabbit PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit before 6 months
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Rabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit PCNA . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PCNA and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit PCNA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit PCNA. The concentration of Rabbit PCNA in samples can be calculated by comparing the OD of the samples to the standard curve.