3h
cell
28pg/mL
leukemia
Sandwich
5000pg/mL
78-5000pg/mL
Rattus norvegicus
B-Cell CLL/Lymphoma 2
Signal transduction;Apoptosis;
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
For cells, cell lines and tissues in culture till half confluency.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Leukemia/Lymphoma 2 (Bcl2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Leukemia/Lymphoma 2 (Bcl2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.