3h
cell
6.5pg/mL
Sandwich
1000pg/mL
Mus musculus
15.62-1000pg/mL
CD31; EndoCAM; GPIIA'; PECA1
CD & Adhesion molecule;Infection immunity;
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Mouse PECAM1(Platelet/Endothelial Adhesion Molecule) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet/Endothelial Cell Adhesion Molecule (PECAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet/Endothelial Cell Adhesion Molecule (PECAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet/Endothelial Cell Adhesion Molecule (PECAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet/Endothelial Cell Adhesion Molecule (PECAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.