Kit
41116158
Colorimetric
AFB standard included
Pathogen and Toxin Assays
2 parts: 1 RT / 1 Blue Ice
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Detection of total Aflatoxin B1 and Aflatoxin B2 adducts in protein samples
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
(AKR-350) GHS.pdf
Upon receipt, aliquot and store the AFB1-BSA Standard and AFB1 Conjugate at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
Cell Biolabs’ Aflatoxin Competitive ELISA Kit provides a convenient method for the detection of total Aflatoxin B1 and Aflatoxin B2 adducts in protein samples. First, an AFB1 conjugate is coated on an ELISA plate.  The unknown AFB1/2 samples or AFB1-BSA standards are then added to the AFB1 conjugate preabsorbed ELISA plate.  After a brief incubation, an anti-AFB1/2 monoclonal antibody is added, followed by an HRP conjugated secondary antibody.  The total content of AFB1 plus AFB2 in unknown samples is determined by comparison with a predetermined AFB1-BSA standard curve.