Kit
cell
41116158
Room Temp
Colorimetric
Cell Based Assays
BrdU standard included
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
For cells, cell lines and tissues in culture till half confluency.
Detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Upon receipt, store the 1000X BrdU Solution and Anti-BrdU Monoclonal Antibody at -20ºC. Store all other components at 4ºC.
(CBA-251) GHS.pdf
The CytoSelect™ BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.