Kit
41116158
Oxidative Stress/Damage
BPDE-BSA standard included
2 parts: 1 RT / 1 Blue Ice
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
For detection and quantitation of benzo(a)pyrene diol epoxide (BPDE) protein adducts
Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
(STA-301) GHS.pdf
Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC.  The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody.  The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.