Kit
41116158
Oxidative Stress/Damage
BPDE-DNA standard included
2 parts: 1 RT / 1 Blue Ice
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Suitable for use with DNA samples such as cell or tissue genomic DNA
For detection and quantitation of benzo(a)pyrene diol epoxide (BPDE) DNA adducts
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
(STA-357) GHS.pdf
Upon receipt, aliquot and store the Reduced DNA and BPDE-DNA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
BPDE-DNA standards or unknown DNA samples are adsorbed onto a 96-well DNA high-binding plate. The BPDE-DNA adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE-DNA adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-DNA standards.