Kit
41116158
2 parts: 1 RT / 1 Blue Ice
Cell Signaling and Protein Biology
Raf1 RBD detects only the active form of Ras
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Convenient 96-well format for use with any plate-based luminometer
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
(STA-441) GHS.pdf
Upon receipt, aliquot and store Raf-1 RBD at -80ºC and avoid freeze/thaw. Aliquot and store the Anti-pan-Ras Antibody, Secondary Antibody, GTPγS, and GDP components at -20ºC and avoid freeze/thaw. Store all other components at 4ºC until their expiration dates.
Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.