Kit
41116158
Pathogen and Toxin Assays
2 parts: 1 RT / 1 Blue Ice
Sensitive to as low as 1 ng/mL
Recombinant HCVcAg standard included
Measures the core antigen of Hepatitis C virus
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Antigens are peptides or recombinant or native dependent on the production method.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
(VPK-151) GHS.pdf
Upon receiving, aliquot and store recombinant HCVcAg Standard at -20ºC and avoid freeze/thaw. Store all other components at 4ºC.
Hepatitis C virus (HCV) NS3, NS4, NS genotypes 1, 2, 3, 4, 5, are small (55–65 nm in size), enveloped, positive-sense single-stranded RNA viruses. Hepatitis C virus is the cause of hepatitis C and some cancers such as Liver Cancer (Hepatocellular carcinoma abbreviated HCC) and lymphomas in humans. HCV Core antigens or envelope proteins are used for immunization to obtain diagnostic antibodies.
An anti-HCVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HCV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HCVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HCVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HCV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HCV core antigen and sample concentration is then determined.