murine GDNF
Mus musculus
sf21, S78-I211
heparin or EDTA
31.2pg/ml to 2000pg/ml
Natural and recombinant mouse GDNF
glial cell derived neurotrophic factor
Glial cell line-derived neurotrophic factor
the ELISA does not cross react with any other proteins except from closely related species
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Astrocyte derived trophic factor;ATF;ATF1;ATF2;Glial cell line derived neurotrophic factor;HFB1 GDNF;hGDNF;HSCR3
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
For quantitative measurement of mouse GDNF in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.010 + 0.069 + 0.099 + 0.195 + 0.389 + 0.790 + 1.469 + 2.305
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);138;716;1354;146;727;1379 ELISA standard deviations 6.07;36.52;86.66;10.51;53.8;113.1 CV(%);4.4;5.1;6.4;7.2;7.4;8.2
Sample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse GDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse GDNF standard solution and each sample be measured in duplicate.
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse GDNF antibodies;1 ST0000-10;Lyophilized recombinant mouse GDNF standard;10ng/tube×2 AR1107;Biotinylated anti- mouse GDNF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
GENTAUR's mouse GDNF ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for GDNF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, S78-I211) and your samples are measured with a biotin conjugated polyclonal from goat specific for GDNF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse GDNF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.