57278
15-20
Q9R069
<15pg/ml
murine BCAM
Mus musculus
NSO, E26-A541
heparin or EDTA
156pg/ml to 10000pg/ml
Basal cell adhesion molecule
Natural and recombinant mouse BCAM
basal cell adhesion molecule (Lutheran blood group)
AU ; Bcam ; CD239 ; CD239 antigen ; F8/G253 antigen ; LU ; Lutheran ; MSK19 ; Q9R069
the ELISA does not cross react with any other proteins except from closely related species
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
For quantitative measurement of mouse BCAM in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.020 + 0.084 + 0.155 + 0.277 + 0.494 + 0.888 + 1.520 + 2.123
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);472;758;1380;563;926;1570 ELISA standard deviations 25.49;47.75;93.84;38.84;68.52;128.74 CV(%);5.4;6.3;6.8;6.9;7.4;8.2
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse BCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse BCAM standard solution and each sample be measured in duplicate.
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse BCAM antibodies;1 ST0000-10;Lyophilized recombinant mouse BCAM standard;10ng/tube×2 AR1107;Biotinylated anti- mouse BCAM antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
GENTAUR's mouse BCAM ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for BCAM was used as capure antibodies on the ELISA test plate. The internal standards (Expression system for standard: NSO, Immunogen sequence: E26-A541) and your samples are measured with a biotin conjugated polyclonal from goat specific for BCAM will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse BCAM in your sample the higher the ELISA signal will be compared to this elisa's standard curve.